Vasculitis & polymyalgia rheumatica
Genes deregulated in giant cell arteritis by Nanostring nCounter gene expression profiling in temporal artery biopsies
Authors' conclusion
TABs with TMI had a distinct transcriptome compared with normal TABs and TABs with ILA. The few genes potentially deregulated in ILA were also deregulated in TMI.
Study context
- Population
- Patients with giant cell arteritis (GCA) and non-GCA controls undergoing temporal artery biopsy
- Participants
- 42 / 7 / 7
- Comparison
- GCA temporal artery biopsies (TABs) with transmural inflammation (TMI) and with inflammation limited to adventitia (ILA) vs normal TABs from patients without GCA
- TMIn = 42
- ILAn = 7
- Non-GCA controlsn = 7reference
- Outcome
- Differentially expressed genes (770 immune-related genes) in TABs
Methodological detailsgene expression profiling, NanoString nCounter PanCancer Immune Profiling Panel, formalin-fixed paraffin-embedded TABs
- Gene expression profiling
- NanoString nCounter PanCancer Immune Profiling Panel
- Formalin-fixed paraffin-embedded TABs
Main finding
As reported in the abstract
Differentially expressed genes (>2.0 fold change, adjusted p<0.05)
- Unsupervised clustering: normal TABs and TABs with ILA in one group, TABs with TMI in the other
- 41/42
- TMI vs normal TABs: downregulated genes
- 31
- TMI vs normal TABs: upregulated genes
- 256
- TMI vs ILA: downregulated genes
- 26
- TMI vs ILA: upregulated genes
- 187
- ILA vs normal TABs: genes with >2.0 fold changes, not significant after Benjamini-Yekutieli correction
- 38
Original publication
Genes deregulated in giant cell arteritis by Nanostring nCounter gene expression profiling in temporal artery biopsies. RMD Open. Published 24 September 2024. doi:10.
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