Lupus & antiphospholipid syndrome
Differential expression pattern of Bcl-2 family members in B and T cells in systemic lupus erythematosus and rheumatoid arthritis
Authors' conclusion
Our study provides evidence for the increased differential expression pattern of Bcl-2 family members in B and T cell subsets of patients with SLE compared to controls.
Study context
- Population
- Patients with systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA) and healthy controls; B and T cell subsets
- Participants
- 14 / 12 / 13
- Comparison
- SLE and RA patients vs healthy controls
- SLEn = 14
- RAn = 12
- Healthy controlsn = 13reference
- Outcome
- Expression of anti-apoptotic Bcl-2 family proteins (Bcl-2, Bcl-XL, Mcl-1) in B and T cells, in relation to disease activity, and effect of BH3 mimetics
Methodological detailsstimulation assays, intracellular flow cytometry, cell viability analyses
- Stimulation assays
- Intracellular flow cytometry
- Cell viability analyses
Main finding
As reported in the abstract
- SLE: decreased induction of Bcl-XL in B cells upon CpG stimulation vs controls
- p ≤ 0.05
- SLE: decreased induction of Mcl-1 in B cells upon CpG stimulation vs controls
- p ≤ 0.001
Further findings
Effect of BH3 mimetics on cell viability
- After CpG stimulation, plasmablasts more viable after 3 BH3 mimetics than naïve or memory B cells, in control and patient cells
- After activation, Mcl-1 inhibition most effective in reducing plasmablast and T cell viability, but less in patients than controls
Original publication
Differential expression pattern of Bcl-2 family members in B and T cells in systemic lupus erythematosus and rheumatoid arthritis. Arthritis Res Ther. Published 22 November 2023. doi:10.
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