Lupus & antiphospholipid syndrome
Clinical value of simultaneous detection of anti-dsDNA and anti-chromatin antibodies using a multiplex immunoassay in systemic lupus erythematosus: a 5-year retrospective study
Authors' conclusion
Concomitant measurement of anti-dsDNA and anti-chromatin antibodies using a multiplex immunoassay system brings complementary information for diagnosis and monitoring of SLE. Both antibodies are associated with disease activity and renal involvement, while anti-chromatin antibodies are associated with neuropsychiatric flares.
Study context
- Population
- Adults with systemic lupus erythematosus (SLE) (2012 SLICC or 2019 EULAR/ACR criteria) followed in a university hospital 2015–2019, with ≥1 positive anti-dsDNA and/or anti-chromatin result and clinical data within 30 days of testing677 antibody evaluations
- Participants
- 98 adults
- Exposure
- Anti-dsDNA vs anti-chromatin antibodies measured concurrently by multiplex immunoassay
- Outcome
- Association of anti-dsDNA and anti-chromatin antibodies with organ-specific manifestations and disease activity (SLEDAI-2K)
Methodological detailssingle-centre (university hospital), mixed models, correlation coefficients
- Single-centre (university hospital)
- Mixed models
- Correlation coefficients
Main finding
As reported in the abstract
Estimate (95% CI)
Anti-dsDNA
OR (cutaneous involvement) 2.29(1.43–3.68)
Anti-dsDNA
OR (musculoskeletal involvement) 1.29(1.02–1.64)
Anti-chromatin
OR (neuropsychiatric manifestations) 1.54(1.03–2.29)
Anti-dsDNAindependently associated
OR (proteinuria >0.5 g/24 hours) 1.17(1.11–2.74)
Anti-chromatinindependently associated
OR (proteinuria >0.5 g/24 hours) 1.71(1.02–2.87)
- Anti-dsDNA · moderate
- r (correlation with SLEDAI-2K) 0.55 (95% CI 0.39–0.67)
- Anti-chromatin · weaker
- r (correlation with SLEDAI-2K) 0.32 (95% CI 0.16–0.51)
Observational data: associations, not effects.
Further findings
| Outcome | R (95% CI) |
|---|---|
| Correlation between anti-dsDNA and anti-chromatin levels | 0.39 (0.21–0.55) |
Discordant results
- Discordant results, patients
- 59 patients (60%)
- Discordant results, assessments
- 215 assessments (32%)
- Anti-chromatin detected without anti-dsDNA
- 111/677 samples (16%)
Original publication
Clinical value of simultaneous detection of anti-dsDNA and anti-chromatin antibodies using a multiplex immunoassay in systemic lupus erythematosus: a 5-year retrospective study. Lupus Sci Med. Published 12 January 2026. doi:10.
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