Spondyloarthritis & psoriatic arthritis
CD_99 G1 neutrophils modulate osteogenic differentiation of mesenchymal stem cells in the pathological process of ankylosing spondylitis
Authors' conclusion
Our results revealed the dynamics of cell subpopulations, gene expression and intercellular interactions during AS pathogenesis.
Study context
- Population
- Spinal enthesis of patients with ankylosing spondylitis (AS) and healthy individuals40,065 single cells profiled
- Participants
- 5 vs 3
- Comparison
- AS vs healthy individuals
- ASn = 5
- Healthy individualsn = 3reference
Methodological detailssingle-cell RNA sequencing, bioinformatics with experimental validation
- Single-cell RNA sequencing
- Bioinformatics with experimental validation
Main finding
As reported in the abstract
- Cells divided into 7 clusters: neutrophils, monocytic cells, granulomonocytic progenitor_erythroblasts, T cells, B cells, plasma cells and stromal cells
- Two differentiation directions of stromal cells from MSC subpopulation MSC-C2 to two Cxcl12-abundant-reticular (CAR) cell subsets, Osteo-CAR and Adipo-CAR
- Transcription factors C-JUN, C-FOS and CAVIN1 highly expressed in AS and regulated osteogenesis of MSCs
- Novel subcluster of early-stage neutrophils, CD99_G1, elevated in AS
- Ligand-receptor mapping revealed recruitment of CD99_G1 neutrophils by Adipo-CAR cells and the pathogenesis of osteogenesis induced in AS
Original publication
CD_99 G1 neutrophils modulate osteogenic differentiation of mesenchymal stem cells in the pathological process of ankylosing spondylitis. Ann Rheum Dis. Published 15 February 2024. doi:10.
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