A novel long non-coding RNA, lnc-RNU12, influences the T-cell cycle via c-JUN and CCNL2 in rheumatoid arthritis
Authors' conclusion
Our study suggested that lnc-RNU12 was involved in the pathogenesis of RA by influencing the T cell cycle by targeting c-JUN and CCNL2.
Study context
- Population
- PBMCs from RA cases and controls; Jurkat T cell lines
- Participants
- 28 vs 18
- Comparison
- RA cases vs controls
- RAn = 28
- Controlsn = 18reference
Methodological detailsgenome-wide lncRNA microarray screening, knock-down in Jurkat T cell lines
- Genome-wide lncRNA microarray screening
- Knock-down in Jurkat T cell lines
Main finding
As reported in the abstract
- lnc-RNU12 downregulated in PBMCs and T cell subtypes of RA patients and genetically associated with RA risk
- lnc-RNU12 mediates the effect of microbiome alterations on RA risk
- Activation of T cells caused low expression of lnc-RNU12
- Knock-down of lnc-RNU12 in Jurkat T cells caused cell cycle S-phase arrest and altered expression of cell cycle- and apoptosis-related genes (e.g. c-JUN, CCNL2, CDK6, MYC, RNF40, PKM, VPS35, DNAJB6, FLCN)
- c-JUN and CCNL2 identified as target genes of lnc-RNU12 at mRNA and protein levels; RNA-binding protein immunoprecipitation verified interaction with c-Jun and cyclin L2 in Jurkat cells
Original publication
A novel long non-coding RNA, lnc-RNU12, influences the T-cell cycle via c-JUN and CCNL2 in rheumatoid arthritis. Rheumatology (Oxford). Published 27 September 2022. doi:10.
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