Lupus & antiphospholipid syndrome
3D genome alterations in T cells associated with disease activity of systemic lupus erythematosus
Authors' conclusion
Our results highlight the 3D genome structure alterations associated with SLE development
Study context
- Population
- Patients with systemic lupus erythematosus (SLE) and age-matched and sex-matched healthy controls; CD4+ T cells
- Participants
- 7 vs 5
- Comparison
- CD4+ T cells of patients with SLE vs age-matched and sex-matched healthy controls (HCs)
- SLEn = 7
- HCsn = 5reference
- Outcome
- 3D genome structure in CD4+ T cells
Methodological detailsHi-C, RNA sequencing, age-matched and sex-matched controls, Integrative analyses: transcription factor motif enrichment, SPI1 knockdown, histone modifications (H3K27ac, H3K4me1, H3K4me3)
- Hi-C
- RNA sequencing
- Age-matched and sex-matched controls
- Integrative analyses: transcription factor motif enrichment, SPI1 knockdown, histone modifications (H3K27ac, H3K4me1, H3K4me3)
Main finding
As reported in the abstract
- 3D chromosome organisation in SLE T cells clearly distinct from HCs and closely associated with SLE disease activity
- Loops within chromosomes associated with SLE disease activity and differentially expressed genes; key histone modifications close to these loops
- Transcription factor SPI1, whose motif is in the altered loop in SLE, contributes to overexpression of interferon pathway gene
- Potential influences of genetic variations in 3D genome alterations in SLE
Original publication
3D genome alterations in T cells associated with disease activity of systemic lupus erythematosus. Ann Rheum Dis. Published 1 September 2022. doi:10.
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