Systemic sclerosis & Raynaud's
The role of iTr35 cells in the inflammatory response and fibrosis progression of systemic sclerosis
Authors' conclusion
Both iTr35 and Tr1 cells are involved in SSc-related inflammation and fibrosis. IL-35 can induce iTr35 cells, showing a synergistic effect with IL-10. We also found that iTr35 cells can inhibit T cell proliferation and differentiation via the STAT3/6 signalling pathway, thereby causing fibrosis.
Study context
- Population
- Patients with SSc
- Participants
- 68 patients
- Comparison
- SSc patients vs control group
- SScn = 68
Methodological detailsflow cytometry, ELISA, immunofluorescence, western blot and immunohistochemistry
- Flow cytometry
- ELISA
- Immunofluorescence, western blot and immunohistochemistry
Main finding
As reported in the abstract
- Peripheral blood: iTr35 cell proportions higher and Tr1 cells lower than control
- IL-35 expression increased, IL-10 levels decreased
- Skin fibroblasts: EBI3, IL-12Ap35, Foxp3 and IL-10 decreased; collagen I, TGF-β, α-SMA and fibronectin increased
- Phosphorylated STAT3/6 increased; iTr35 and Tr1 cell levels significantly decreased
- CD4+ cells with both rhIL-35 and rhIL-10: more iTr35 and Tr1 cells than with rhIL-35 or rhIL-10 alone
- Viability of conventional CD4+ T cells decreased with increasing iTr35 cells
- iTr35 cells affected α-SMA expression through the STAT3/6 signalling pathway
Original publication
The role of iTr35 cells in the inflammatory response and fibrosis progression of systemic sclerosis. Rheumatology (Oxford). Published 3 February 2023. doi:10.
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