Th17/1 and ex-Th17 cells are detected in patients with polyarticular juvenile arthritis and increase following treatment
Authors' conclusion
Patients with polyJIA have increased baseline Th17 cells, potentially reflecting higher tonic STAT3 activation in vivo.
Study context
- Population
- Patients with polyarticular JIA (polyJIA), treatment-naïve at diagnosis, and pediatric healthy controls
- Comparison
- Treatment-naïve polyJIA patients vs pediatric healthy controls; polyJIA patients post-treatment (remission) vs at diagnosis
- polyJIAn = 17
- Outcome
- Th17 cells and STAT3 activation in CD4+ T cells (flow cytometry of PBMCs)
Methodological detailspaired pre- and post-treatment sampling, flow cytometry, Mann-Whitney U and Wilcoxon matched-pairs signed rank tests
- Paired pre- and post-treatment sampling
- Flow cytometry
- Mann-Whitney U and Wilcoxon matched-pairs signed rank tests
Main finding
As reported in the abstract
Th17 cells (CD3+CD4+IL-17+), treatment-naïve polyJIA vs controls · initial diagnosis
- Th17 cells increased in treatment-naïve polyJIA vs controls (0.15% v 0.44%), p < 0.05
- Tregs (CD3+CD4+CD25+FOXP3+) did not differ from controls
- Changes in STAT3 phosphorylation in CD4+ T cells after ex vivo stimulation not significantly different vs controls
- Dual IL-17+ and IFNγ+ CD4+ T cells identified in patients, but not controls
Further findings
Th17/1 cells (CCR6+CD161+IFNγ+IL-17+) in polyJIA post-treatment · post-treatment (remission)
- Increased in patients post-treatment, p < 0.05
- 0.3% v 0.07%
Ex-Th17 cells (CCR6+CD161+IFNγ+IL-17neg) in polyJIA post-treatment · post-treatment (remission)
- Increased in patients post-treatment, p < 0.05
- 2.3% v 1.4%
IL-17-expressing cells post-treatment and therapy dependence
- Patients with the highest IL-17 expressing cells post-treatment remained therapy-bound
Original publication
Th17/1 and ex-Th17 cells are detected in patients with polyarticular juvenile arthritis and increase following treatment. Pediatr Rheumatol Online J. Published 2 March 2024. doi:10.
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