Serum cholesterol loading capacity of macrophages is regulated by seropositivity and C-reactive protein in rheumatoid arthritis patients
Authors' conclusion
Oxidized LDL can directly influence CLC in dual-seropositive RA patients. Two additional and independent pathways-via anti-oxLDL IgG and PCSK9-may mediate the effects of oxPL-apoB100 on CLC, depending on CRP and seropositivity status.
Study context
- Population
- Patients with RA; serum incubated with human THP-1-derived macrophages
- Participants
- 104 patients
- Outcome
- CLC of macrophages (intracellular cholesterol content after incubation with patient serum)
Methodological detailsobservational cohort, mediation and moderation analysis
- Observational cohort
- Mediation and moderation analysis
Main finding
As reported in the abstract
Factors associated with CLC
- OxPL-apoB100, anti-oxLDL IgG and PCSK9 positively associated with cholesterol loading capacity (CLC)
- all P < 0.020
- Direct effect of oxPL-apoB100 on CLC only in dual RF- and ACPA-positive patients, unstandardized b
- 2.08 (0.38, 3.79) (95% bootstrap CI)
- Indirect effect of oxPL-apoB100 on CLC via anti-oxLDL IgG increased with CRP level, index of moderated mediation
- 0.55 (0.05-1.17)
- CRP-moderated indirect effect of oxPL-apoB100 on CLC via PCSK9 upregulation, only in dual-seropositive patients, conditional indirect effect
- 0.64 (0.13-1.30)
Observational data: associations, not effects.
Original publication
Serum cholesterol loading capacity of macrophages is regulated by seropositivity and C-reactive protein in rheumatoid arthritis patients. Rheumatology (Oxford). Published 9 July 2022. doi:10.
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