Profiling the peripheral immune response to ex vivo TNF stimulation in untreated juvenile idiopathic arthritis using single cell RNA sequencing
Authors' conclusion
These results support the development of personalized immune-profiling combined with ex-vivo immune stimulation for evaluation of patient-specific modes of immune cell activity in autoimmune rheumatic disease.
Study context
- Population
- Untreated children newly diagnosed with Juvenile Idiopathic Arthritis (JIA) and healthy controls; whole blood cultured 24 h with or without ex vivo TNF stimulation
- Participants
- 6 vs 2
- Comparison
- Untreated newly diagnosed JIA vs healthy controls; ex vivo TNF stimulation vs no stimulation
- JIAn = 6
- Healthy controlsn = 2reference
- Outcome
- Immune cell-types and transcriptional responses to TNF stimulation (scRNAseq)
- Follow-up
- 24 h
Methodological detailsproof-of-concept, single-cell RNA sequencing, ex vivo TNF stimulation, Novel analytical pipeline scPool: cells pooled into pseudocells before expression analysis, allowing variance partitioning of TNF stimulus, JIA status and donor effects
- Proof-of-concept
- Single-cell RNA sequencing
- Ex vivo TNF stimulation
- Novel analytical pipeline scPool: cells pooled into pseudocells before expression analysis, allowing variance partitioning of TNF stimulus, JIA status and donor effects
Main finding
As reported in the abstract
24 h
Robust immune cell-types identified
17
Original publication
Profiling the peripheral immune response to ex vivo TNF stimulation in untreated juvenile idiopathic arthritis using single cell RNA sequencing. Pediatr Rheumatol Online J. Published 15 February 2023. doi:10.
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