Lupus & antiphospholipid syndrome
Phospholipase D4 as a signature of toll-like receptor 7 or 9 signaling is expressed on blastic T-bet + B cells in systemic lupus erythematosus
Authors' conclusion
PLD4 + B cells, especially "blastic" ones, are likely autoreactive B cells undergoing TLR stimulation.
Study context
- Population
- Peripheral blood mononuclear cells (PBMCs) from healthy donors and patients with systemic lupus erythematosus (SLE)
- Participants
- 23 vs 40
- Comparison
- Patients with SLE vs healthy donors
- Healthy donors (HDs)n = 23reference
- SLEn = 40
- Outcome
- Frequency of PLD4 + B cells
Methodological detailsflow cytometry, in vitro cell culture, recombinant antibody synthesis
- Flow cytometry
- In vitro cell culture
- Recombinant antibody synthesis
Main finding
As reported in the abstract
P < 0.005
- Frequencies of phospholipase D4 (PLD4) + blasts significantly correlated with those of plasmablasts
- P < 0.005
- Recombinant antibodies from PLD4 + blasts with antinuclear activity
- two out of three
Original publication
Phospholipase D4 as a signature of toll-like receptor 7 or 9 signaling is expressed on blastic T-bet + B cells in systemic lupus erythematosus. Arthritis Res Ther. Published 16 October 2023. doi:10.
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