Lupus & antiphospholipid syndrome
Oxysterols contribute to immune cell recruitment in systemic lupus erythematosus (SLE) skin lesions
Authors' conclusion
Our findings reveal a potential mechanism by which fibroblasts contribute to immune cell recruitment in SLE skin lesions by expression of CH25H and CYP7B1.
Study context
- Population
- SLE skin lesions
Methodological detailstargeted oxidized lipid metabolomics (liquid chromatography-mass spectrometry (LC-MS)), immunohistochemical staining, single-cell sequencing data analysis, transwell chemotaxis assay
- Targeted oxidized lipid metabolomics (LC-MS)
- Immunohistochemical staining
- Single-cell sequencing data analysis
- Transwell chemotaxis assay
Main finding
As reported in the abstract
- Aberrant oxidized cholesterol metabolism in SLE skin lesions, with elevated 7-ketocholesterol, 5α-6α-cholestane-3β,5α,6β-triol and others
- Fibroblasts were the primary cells expressing oxysterol-encoding genes; CH25H and CYP7B1 upregulated via IL-1β-mediated p38 MAPK and NFκB pathways
- IL-1β-stimulated fibroblasts showed enhanced PBMC recruitment, attenuated by a GPR183 inhibitor
Original publication
Oxysterols contribute to immune cell recruitment in SLE skin lesions. Arthritis Res Ther. Published 22 October 2024. doi:10.
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