Lupus & antiphospholipid syndrome
Outgrowth of Escherichia is susceptible to aggravation of systemic lupus erythematosus
Authors' conclusion
We characterize a novel SLE exacerbating Escherichia outgrowth and suggest its contribution to SLE procession may be partially associated with metabolite changes and cross-reactivity of gut microbiota-associated epitopes and host autoantigens.
Study context
- Population
- Systemic lupus erythematosus (SLE) patients and healthy controls undergoing faecal gut microbiome sequencing; lupus-prone MRL/lpr mice16S ribosomal RNA (rRNA) sequencing and shotgun metagenomics cohorts
- Comparison
- SLE cases vs healthy controls (HCs); faecal Escherichia gavage in MRL/lpr mice
- SLEn = 114 for 16S rRNA sequencing; 124 for shotgun metagenomics
- HCsn = 134 for 16S rRNA sequencing; 97 for shotgun metagenomicsreference
- Outcome
- SLE-pathogenic gut microbes and their potential mechanisms
Methodological details16S rRNA amplicon sequencing, shotgun metagenomics, oral gavage into lupus-prone MRL/lpr mice
- 16S rRNA amplicon sequencing
- Shotgun metagenomics
- Oral gavage into lupus-prone MRL/lpr mice
Main finding
As reported in the abstract
- Gut microbiota richness reduction and composition alteration in SLE patients, particularly LN and active patients
- P/B ratio remarkably up-regulated
- Escherichia identified as the dominantly expanded genus in SLE; metagenomics accurately located Escherichia coli and Escherichia unclassified species
- Significant associations among Escherichia coli, metabolic pathways of purine nucleotide salvage or peptidoglycan maturation and SLE disease activity index (SLEDAI)
- Associations between multiple epitopes from Escherichia coli and disease activity or renal involvement phenotype
- Gavage with faecal Escherichia upregulated lupus-associated serum traits and aggravated glomerular lesions in MRL/lpr mice
Observational data: associations, not effects.
Original publication
Outgrowth of Escherichia is susceptible to aggravation of systemic lupus erythematosus. Arthritis Res Ther. Published 7 November 2024. doi:10.
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