Sjögren's disease & overlap diseases
Multimodal Single‐Cell Sequencing of B Cells in Primary Sjögren's Syndrome
Authors' conclusion
We describe heterogeneity and molecular characteristics in B cells from patients with pSS, providing clues to intrinsic differences in B cells that affect the phenotype and outcome and allowing stratification of patients with pSS at improved resolution.
Study context
- Population
- Peripheral blood B cells (over 230,000) from patients with pSS and healthy controlsOver 230,000 B cells
- Participants
- 6 / 8 / 10 / 4
- Comparison
- pSS subgroups stratified by SSA/SSB antibodies vs healthy controls
- SSA-n = 6
- SSA+n = 8
- SSA/SSB+n = 10
- Healthy controlsn = 4reference
Methodological detailssingle-cell RNA sequencing, single-cell variable, diversity, and joining (VDJ) sequencing
- Single-cell RNA sequencing
- Single-cell VDJ sequencing
Main finding
As reported in the abstract
- SSA/SSB+ patients had the highest proportion of naïve and lowest proportion of memory B cells
- SSA/SSB+ patients had the highest up-regulation of interferon-induced genes across all B cell subtypes
- IGHV1-69 and IGHV4-30-4 more often used in all pSS subgroups vs controls
- Memory B cells from SSA/SSB+ patients had a higher proportion of unmutated VDJ transcripts vs other pSS groups and controls, indicating altered somatic hypermutation
- Heterogeneous clonotype pools with little overlap in CDR3 sequences vs previous studies
- Joint single-cell RNA sequencing (scRNA-seq) and scVDJ-seq analysis allowed unsupervised stratification of pSS and identified novel parameters correlated to disease manifestations and antibody status
Original publication
Multimodal Single‐Cell Sequencing of B Cells in Primary Sjögren's Syndrome. Arthritis Rheumatol. Published 18 December 2023. doi:10.
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