Lupus & antiphospholipid syndrome
Multimodal repertoire analysis unveils B cell biology in immune-mediated diseases
Authors' conclusion
Our multimodal repertoire analysis enabled us the system-level understanding of B cell abnormality in diseases.
Study context
- Population
- Donors with immune-mediated diseases and healthy donors; five sorted B cell subsetsImmune-mediated diseases (IMDs) and health
- Participants
- 595 donors
Methodological detailsmultimodal B cell receptor repertoire analysis, RNA sequencing of sorted B cell subsets
- Multimodal B cell receptor repertoire analysis
- RNA sequencing of sorted B cell subsets
Main finding
As reported in the abstract
- CDR-H3 length among naïve B cells shortened in autoimmune diseases
- Strong negative correlation between interferon signature strength and CDR-H3 length in naïve B cells
- VDJ gene usage skewed especially in plasmablasts and unswitched-memory B cells of patients with systemic lupus erythematosus (SLE)
- Skewing score correlated positively with peripheral helper T cell transcriptomic signatures and negatively with somatic hypermutation in plasmablasts
- High IGHV4-34 usage with 9G4 idiotypes in unswitched-memory B cells, with prominent positive correlation with SLE disease activity
- Gene usage skewing in unswitched-memory B cells ameliorated after belimumab treatment
Original publication
Multimodal repertoire analysis unveils B cell biology in immune-mediated diseases. Ann Rheum Dis. Published 19 July 2023. doi:10.
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