Lupus & antiphospholipid syndrome
Mononuclear cells from patients with rhupus are influenced by tumour necrosis factor (TNF) in their production of gp130/sIL-6Rb and a proliferation-inducing ligand (APRIL)
Authors' conclusion
These findings suggest that the spontaneous production of molecules by cells from patients with rhupus may contribute to the development of the disease, and that TNF may play a role in this process by regulating the secretion of gp130/sIL-6Rb and APRIL.
Study context
- Population
- Patients with rhupus, patients with systemic lupus erythematosus (SLE) and healthy controls; Peripheral blood mononuclear cells (PBMCs)
- Participants
- 5 / 7 / 7
- Comparison
- TNF-stimulated vs unstimulated PBMCs from patients with rhupus, SLE and healthy controls
- Healthy controlsn = 5reference
- Rhupusn = 7
- SLEn = 7
- Outcome
- Levels of molecules (cytokines, chemokines) secreted by PBMCs stimulated with recombinant human TNF
- Follow-up
- 24 hours
Methodological detailsin vitro PBMC stimulation with recombinant human TNF for 24 hours, immunobead-based assays on xMAP technology, ELISA validation against rheumatoid arthritis (RA)
- In vitro PBMC stimulation with recombinant human TNF for 24 hours
- Immunobead-based assays on xMAP technology
- ELISA validation against RA
Main finding
As reported in the abstract
Molecules secreted by PBMCs
- Production of some molecules higher in TNF-stimulated than unstimulated PBMCs from rhupus and SLE
- gp130/sIL-6Rb, APRIL, interferon-β, matrix metalloproteinase-3 and IL-12 higher in rhupus PBMCs even without TNF stimulation
- gp130/sIL-6Rb and APRIL higher in TNF-stimulated rhupus PBMCs than in healthy controls
- Results further validated against RA by ELISA
Original publication
Mononuclear cells from patients with rhupus are influenced by TNF in their production of gp130/sIL-6Rb and APRIL. Lupus Sci Med. Published 25 October 2023. doi:10.
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