Methotrexate promotes the release of granulocyte–macrophage colony-stimulating factor from rheumatoid arthritis fibroblast-like synoviocytes via autocrine interleukin-1 signaling
Authors' conclusion
MTX treatment induces secretion of IL-1 from activated RA-FLS which by autocrine signaling augments their release of GM-CSF.
Study context
- Population
- Primary RA-FLS activated with IL-1β or platelet-derived growth factor + IL-1β
- Comparison
- Methotrexate (MTX) or tofacitinib (TOFA) vs no drug in activated RA-FLS
- Outcome
- Gene expression in activated RA-FLS
Methodological detailsin vitro, RNA sequencing, co-culture
- In vitro
- RNA sequencing
- Co-culture
Main finding
As reported in the abstract
Differentially expressed genes with MTX, majority upregulated
6350
Differentially expressed genes with TOFA, majority downregulated
970
Original publication
Methotrexate promotes the release of granulocyte–macrophage colony-stimulating factor from rheumatoid arthritis fibroblast-like synoviocytes via autocrine interleukin-1 signaling. Arthritis Res Ther. Published 11 October 2024. doi:10.
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