Individual immune cell and cytokine profiles determine platelet-rich plasma composition
Authors' conclusion
Individuals with elevated levels of cells with pro-inflammatory properties maintain these in the final PRP products. The concentration of pro-inflammatory cytokines strongly varies between PRP products.
Study context
- Population
- Healthy donors; Platelet-rich plasma (PRP) products and primary human chondrocyte cultures exposed to recombinant IFN-γ and TNF-α
- Participants
- 12 healthy donors
- Comparison
- Three PRP preparations vs donor blood
Methodological detailsin vitro, flow cytometry, cytokine profiling, primary human chondrocyte 2D and 3D cultures
- In vitro
- Flow cytometry
- Cytokine profiling
- Primary human chondrocyte 2D and 3D cultures
Main finding
As reported in the abstract
- All three PRP products showed elevated portions of leukocytes vs baseline donor blood
- IFN-γ and TNF-α significantly increased in nSTRIDE® APS vs donor blood and other PRP products
- All other cytokines and immune cells of donor blood, incl. pro-inflammatory T cell subsets, maintained in all PRP products
- Chondrocyte proliferation impaired by IFN-γ and enhanced by TNF-α
- Differentiation and cartilage formation compromised by both cytokines: altered messenger ribonucleic acid (mRNA) expression of COL1A1, COL2A1 and ACAN, reduced proteoglycan content
Original publication
Individual immune cell and cytokine profiles determine platelet-rich plasma composition. Arthritis Res Ther. Published 10 January 2023. doi:10.
Composed by AI from the published abstract and checked against it. How Briefs are made →