Improved Detection of Myositis‐Specific Autoantibodies Using Luciferase Immunoprecipitation Systems Assay: Comparison With Line Blot and Conventional Immunoprecipitation
Authors' conclusion
LIPS provides quantitative discrimination, strong concordance with IP, and improved performance compared with line blot, particularly at low to intermediate titers.
Study context
- Population
- Sera with line blot results from the Johns Hopkins Myositis Center (IIM)sera
- Participants
- 279 participants
- Comparison
- Luciferase immunoprecipitation systems (LIPS) assay vs line blot, against conventional immunoprecipitation (IP)
- IPn = 141reference
- Outcome
- Performance vs IP
Methodological detailssingle-centre, reference standard: IP
- Single-centre
- Reference standard: IP
Performance
As reported in the abstract
| Test or model | κ | AUC | Sensitivity | Specificity |
|---|---|---|---|---|
| LIPS | 0.90 | 0.935 | ||
| Line blot ≥36 AU | 92.9% | 73.8% | ||
| Line blot ≥71 AU | 82.8% | 92.9% |
κ
LIPS0.90
Line blot ≥36 AU— not stated in the abstract
Line blot ≥71 AU— not stated in the abstract
AUC
LIPS0.935
Line blot ≥36 AU— not stated in the abstract
Line blot ≥71 AU— not stated in the abstract
Sensitivity
LIPS— not stated in the abstract
Line blot ≥36 AU92.9%
Line blot ≥71 AU82.8%
Specificity
LIPS— not stated in the abstract
Line blot ≥36 AU73.8%
Line blot ≥71 AU92.9%
Further findings
| Outcome | Line blot ≥36 AU | Line blot ≥71 AU |
|---|---|---|
| Concordance LIPS vs line blot in patients without IP | 89% | 83% |
- Concordance LIPS vs line blot in patients without IP: 138 patients without IP
Concordance LIPS vs line blot in patients without IP
- LIPS-positive cases enriched for ASyS and DM
Discordant cases (line blot positive, LIPS negative) classified as IBM
- IBM among discordant cases
- 5 of 11
- Anti-Ro52 discrepancies most frequent at titers <36 AU
Original publication
Improved Detection of Myositis‐Specific Autoantibodies Using Luciferase Immunoprecipitation Systems Assay: Comparison With Line Blot and Conventional Immunoprecipitation. Arthritis Rheumatol. Published 31 March 2026. doi:10.
Composed by AI from the published abstract and checked against it. How Briefs are made →