Immunomodulatory effects of dental pulp stem cells on lymphocytes and monocytes from patients with rheumatoid arthritis
Authors' conclusion
The overexpression of inhibitory immune checkpoints by DPSCs may contribute to their immunomodulatory effects. DPSCs modulated a broader range of cytokines in monocyte supernatants compared to lymphocytes.
Study context
- Population
- Peripheral blood lymphocytes and monocytes from rheumatoid arthritis (RA) patients unresponsive to conventional synthetic disease-modifying anti-rheumatic drugs (csDMARDs)RA patients unresponsive to csDMARDs
- Participants
- 12 participants
- Comparison
- Activated RA lymphocytes or monocytes co-cultured with dental pulp stem cells (DPSCs) vs without DPSCs
- Outcome
- Expression of 7 immune checkpoints on DPSCs (flow cytometry) and 17 cytokines in supernatants
- Follow-up
- 48 hours
Methodological detailsin vitro co-culture, flow cytometry
- In vitro co-culture
- Flow cytometry
Main finding
As reported in the abstract
- Co-culture with CD3/CD28-activated lymphocytes or LPS-activated monocytes increased PD-L1, PD-L2, CD155 and Galectin-9 expression on DPSCs
- Lymphocytes activated with ODN 2006 did not alter immune checkpoint expression
- CD80, CD86 and 4-1BBL expression not induced under any condition
- DPSCs reduced sFas-L, IFNγ, sIL-6RA, MIP-1β, TNFα in supernatants of CD3/CD28-activated lymphocytes
- DPSCs reduced BAFF, sFas-L, IL-1β, sIL-6RA, IL-9, IL-17A, IL-18, IL-23, M-CSF, perforin, TNFα in supernatants of LPS-activated monocytes
- IL-6 and IP-10 increased in all conditions; IL-9 increased in ODN 2006- and CD3/CD28-stimulated lymphocyte cultures
- DPSC effects differed by activation status of lymphocytes and monocytes
Original publication
Immunomodulatory effects of dental pulp stem cells on lymphocytes and monocytes from patients with rheumatoid arthritis. Clin Exp Rheumatol. Published 8 May 2025. doi:10.
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