Assessing the Immune Cell Subset and Genetic Mutations in Patients With Palindromic Rheumatism Seronegative for Rheumatoid Factor and Anti–Cyclic Citrullinated Peptide
Authors' conclusion
In seronegative PR patients, we identified a distinct CD14+CD11b+CD36+ cell subset that can induce an inflammatory response under stress and exert antiinflammatory effects after phagocytosis of apoptotic cells, and a CD4+CD25-CD69+ T cell subset with pro- and antiinflammatory properties.
Study context
- Population
- Patients with seronegative palindromic rheumatism and healthy controls, single centre
- Participants
- 48 vs 48
- Comparison
- Seronegative palindromic rheumatism (PR) vs healthy controls
- Seronegative PRn = 48
- Healthy controlsn = 48reference
- Outcome
- Immune cell subsets and genes in seronegative PR
Methodological detailssingle-centre, comparative, Mass cytometry and RNA sequencing of blood; ELISA of plasma cytokines (40 patients), flow cytometry (25), real-time PCR (21), whole-exome sequencing (3)
- Single-centre
- Comparative
- Mass cytometry and RNA sequencing of blood; ELISA of plasma cytokines (40 patients), flow cytometry (25), real-time PCR (21), whole-exome sequencing (3)
Main finding
As reported in the abstract
- Markedly increased frequency of CD14+CD11b+CD36+ and CD4+CD25-CD69+ cells in seronegative PR patients with active flares vs healthy controls
- Gene enrichment of sorted subsets: inflammatory/stress response, phagocytosis and regulation of apoptosis pathways
- Up-regulated CXCL16 and IL10RA in monocytes from PR patients
- Up-regulation of PFKFB3, DDIT4 and TGFB1, down-regulation of PDIA6 in monocytes and lymphocytes from PR patients with active flares and in intercritical periods
- Elevated plasma S100A8/A9 and IL-1β in PR patients
- Whole-exome sequencing: novel polygenic mutations in HACL1, KDM5A, RASAL1, HAVCR2, PRDM9, MBOAT4 and JRKL
- Seronegative PR vs Healthy controls: P < 0.0001 for both cell subset comparisons
Original publication
Assessing the Immune Cell Subset and Genetic Mutations in Patients With Palindromic Rheumatism Seronegative for Rheumatoid Factor and Anti–Cyclic Citrullinated Peptide. Arthritis Rheumatol. Published 5 January 2023. doi:10.
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